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KMID : 1007520160250030935
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Food Science and Biotechnology
2016 Volume.25 No. 3 p.935 ~ p.940
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Synergistic Antimicrobial Activity of Bacteriophages and Antibiotics against Staphylococcus aureus
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Jo A-Ra
Ding Tian
Ahn Ju-Hee
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Abstract
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This study was designed to assess the synergistic antimicrobial effect of phages combined with antibiotics against Staphylococcus aureus. The phage-antibiotic synergy (PAS) effect was evaluated using the fractional inhibitory concentration (FIC) and flow cytometric analysis. The determined minimum inhibitory concentration (MIC) values varied from 0.125 to 128 μg/mL for S. aureus KACC 13236 (SAS) and from 0.25 to >256 μg/mL for S. aureus CCARM 3080 (SAR). The PAS effect was more pronounced in SAS treated with phage SA11 in the presence of cefoxitin (FIC=0.62), chloramphenicol (FIC=0.54), and polymyxin B (FIC=0.38). SAS and SAR cells were injured when exposed to asublethal concentration of ciprofloxacin, whereas these cells were highly susceptible to the phage-antibiotic combined treatment, showing 96% of relative percentages of injured and dead cells. The results suggest that the combined treatment of phages and antibiotics can be used to improve antimicrobial efficacy against antibiotic-resistant bacteria.
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KEYWORD
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phage-antibiotic synergy, fractional inhibitory concentration, Staphylococcus aureus, antibiotic resistance, bacteriophage
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